THE REACTION OF HUMAN CHORIONIC AND EQUINE GONADOTROPINS TO FORMALDEHYDE

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Abstract
The reaction of mare serum hormone or prolan in 4% formaldehyde conc. at pH 8.5 was terminated either by dilution of the reaction product and immediate dosage or by conversion of the excess formaldehyde to hexamethylene tetramine by ammonia. 1 and 3 hrs.'' exposure to formaldehyde produced progressive partial inactivation of both hormones and within the limitations of the assay to the same degree. 1/4 hr. exposure of mare serum hormone to formaldehyde resulted in almost complete recovery of active material. Under the conditions of the formaldehyde reaction in these expts. no difference in the chemical structures of mare serum hormone and prolan could be detected. There is no indication that the free amino group is essential to mare serum activity and non-essential to prolan activity.

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