Regulation of yeast fructose‐1,6‐bisphosphatase in strains containing multicopy plasmids coding for this enzyme

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Abstract
The structural gene for yeast fructose‐1,6‐bisphosphatase (FbPase) has been cloned by complementation of a strain carrying the fbp1‐1 mutation and lacking FbPase activity. By Northern analysis it could be shown that the fbp1‐1 mutation does not interfere with the transcription of the gene although no normal active enzyme is produced. Strains transformed with the cloned gene on a multicopy plasmid overproduce FbPase but are still subject to strong catabolite repression. Catabolite inactivation, however, is slowed down in such strains.