Arabinosyl Nucleosides Inhibit Toxoplasma gondii and Allow the Selection of Resistant Mutants

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Abstract
Adenine arabinoside which inhibits the synthesis of DNA by intracellular T. gondii, is more inhibitory to the parasite than it is to cultured human fibroblast host cells. A single-step mutant of T. gondii that is 50-fold more resistant to adenine arabinoside was isolated after mutagenesis with N-methyl-N''-nitro-N-nitrosoguanidine. Cytosine arabinoside is notably more inhibitory to cultured human cells than it is to T. gondii. In infected cells treated with cytosine arabinoside and labeled with 3H-deoxyuridine nearly all of the label is specifically incorporated into the intracellular T. gondii.