Mapping of Myxococcus xanthus Social Motility dsp Mutations to the dif Genes
Open Access
- 1 March 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (5) , 1462-1465
- https://doi.org/10.1128/jb.184.5.1462-1465.2002
Abstract
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes. Another dsp allele contains a mutation in the sglK gene. About 36.6 kb around the dsp-dif locus was sequenced and annotated, and 50% of the genes are novel.Keywords
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