Stimulation of Locomotion of Peripheral Blood Monocytes by Human Plasma Fibronectin

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Abstract
The motility of human peripheral blood granulocytes and monocytes in response to human plasma fibronectin was quantified by an in vitro assay blind-well chemotaxis chambers. Purified fibronectin under nondenaturing conditions produced increased migration of granulocytes only at concentrations > 100 nM, and induced increased chemotactic and random locomotion of monocytes at concentrations > 0.1 nM. The monocyte migration-inducing activity of fibronectin was concentration dependent, and was strongly inhibited by low concentrations of colchicine (100 nM-100 .mu.M). Plasma fibronectin apparently serves as a chemotactic stimulus for monocytes in vivo and attracts these cells to sites of microscopic tissue injury where plasma fibronectin is deposited.