The Use of a Bile-Aesculin Medium and of Maxted's Technique of Lancefield Grouping in the Identification of Enterococci (Group D Streptococci)

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Abstract
117 Lancefield group D strains were found to be heat-resistant, bile-tolerant and esculin-hydrolyzing. Of 25 alpha-hemolytic streptococci, not of group D, none hydrolyzed esculin but 2 were heat-resistant and 2 were bile-tolerant. From the high correlation between Lancefield grouping and hydrolysis of esculin in bile containing medium, the latter is considered as a valid test for recognition of enterococci.