Simple Measurement of Captopril in Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection
- 1 January 1990
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 12 (1) , 105-110
- https://doi.org/10.1097/00007691-199001000-00019
Abstract
Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10–1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method.This publication has 9 references indexed in Scilit:
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