A Hidden Antigenic Site Localized to the Constant Region of Light Chains of Immunoglobulins
- 9 February 1973
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 179 (4073) , 580-582
- https://doi.org/10.1126/science.179.4073.580
Abstract
An antigenic site, which was not immunochemically demonstrable in the intact κ light polypeptide chain, was exposed by enzymatic cleavage of the κ chain into its variant half and constant half. This antigenic site located in the constant region the κ chain was detected immunochemically by several antiserums that had specificity for this site. Treatment of an intact κ chain with a dissociating agent resulted in the exposure of the hidden antigenic site, which was as readily detected in the unfolded polypeptide chain as in the isolated constant half.Keywords
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