Site-directed mutagenesis in the active site of the herpes simplex virus type 1 thymidine kinase gene
- 1 June 1993
- journal article
- Published by Springer Nature in Virus Genes
- Vol. 7 (2) , 205-209
- https://doi.org/10.1007/bf01702400
Abstract
The thymidine kinase (TK) of herpes simplex virus type 1 (HSV-1) contains three regions of homology to other ATP utilizing enzymes. We have altered one region of the protein, which seems to play an important role in phosphorylating substrates by site-directed mutagenesis. When the aspartate 162 was changed to asparagine, the enzyme lost its activity. To identify the inactive protein, expressed by a vaccinia vector in eukaryotic cells, a monospecific antiserum against a bacterial tryptophan E-HSV-1 TK fusion protein was made. These results support the suggestion that aspartate 162 is essential for the enzymatic activity.Keywords
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