Effects of FSH on Cyclic Nucleotide Accumulation in Testes of Rats of Various Ages

Abstract
The accumulation of cyclic AMP in rat testis in response to FSH is much greater in immature raf compared to adult. Addition of 3-isobutyl-l-methylxanthine (MIX), a potent in vitro inhibitor of cyclic nucleotide phosphodiesterase activity, potentiates the effects of FSH but does not restore the cyclic AMP response of adult rat to that of immature rat testis. The apparent substrate affinities for cyclic AMP and cyclic GMP hydrolysis are similar in immature and mature testis. Developmental changes in cyclic AMP and cyclic GMP phosphodiesterase specific activities fail to account for the reduced responsiveness of adult testis to FSH. These results suggest a defect in synthetic rather than hydrolytic mechanisms in FSH-responsive cells.