Demonstration of Alkaline Phosphatase and Periodic Acid-Schiff Positive Material in the Same Section
- 1 January 1958
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 33 (5) , 225-228
- https://doi.org/10.3109/10520295809111854
Abstract
Alkaline phosphatase activity and periodic acid-Schiff (PAS) positive material were demonstrated in the same section by producing a gold-toned silver deposit at the sites of enzyme activity, followed by the PAS reaction. The glycerophosphate incubating mixture was modified by substituting AgNO3 (final concentration about 0.17%) for the magnesium salt, and, after a slightly lengthened incubation, the silver was reduced by means of a fine grain photographic developer. Gold toning was followed by the regular PAS staining procedure. Gold deposits in the sites of enzyme activity were fully resistant to the action of periodic acid.Keywords
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