Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling
- 1 September 1997
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 4 (9) , 694-699
- https://doi.org/10.1038/nsb0997-694
Abstract
The solution structure of the Ras-binding domain (RBD) of Ral guanine-nucleotide exchange factor RalGEF was solved by NMR spectroscopy. The overall structure is similar to that of Raf-RBD, another effector of Ras, although the sequence identity is only 13%. 1SN chemical shifts changes in the complex of RalGEF-RBD with Ras indicate an interaction similar to the intermolecular β-sheet observed for the complex between Ras and Raf-RBD.Keywords
This publication has 30 references indexed in Scilit:
- Molecular Studies of CtpA, the Carboxyl-terminal Processing Protease for the D1 Protein of the Photosystem II Reaction Center in Higher PlantsJournal of Biological Chemistry, 1996
- Interactions between Ras proteins and their effectorsCurrent Opinion in Biotechnology, 1996
- Ras/Rap effector specificity determined by charge reversalNature Structural & Molecular Biology, 1996
- Differential Interaction of the Ras Family GTP-binding Proteins H-Ras, Rap1A, and R-Ras with the Putative Effector Molecules Raf Kinase and Ral-Guanine Nucleotide Exchange FactorPublished by Elsevier ,1996
- MOLMOL: A program for display and analysis of macromolecular structuresJournal of Molecular Graphics, 1996
- Conformational Transitions in p21ras and in Its Complexes with the Effector Protein Raf-RBD and the GTPase Activating Protein GAPBiochemistry, 1996
- The 2.2 Å crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with RaplA and a GTP analogueNature, 1995
- Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membraneNature, 1994
- The GTPase superfamily: conserved structure and molecular mechanismNature, 1991
- Determination of three‐dimensional structures of proteins from interproton distance data by hybrid distance geometry‐dynamical simulated annealing calculationsFEBS Letters, 1988