Identification of positional isomers of lysophosphatides in butter serum

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Abstract
The separation of individual classes of phospholipids by two‐dimensional thin layer chromatography revealed the presence of lysophosphatides (monoacyl glycerophosphoryl compounds) in minor quantities in butter serum. The naturally occurring lysophosphatidyl ethanolamine (LPE) and lysophosphatidyl choline (LPC) were prepared by the combined use of a diethylaminoethyl (DEAE) cellulose column and thin layer chromatography. Gas chromatographic analysis of methyl esters of LPE and LPC showed that both lysophosphatides contained, in addition to saturated fattya cids, considerable quantities of unsaturated fatty acids. A comparison of the fatty acid pattern of naturally occurring LPE and LPC with that of LPE and LPC prepared by phospholipase A hydrolysis of phosphatidyl ethanolamine (PE) and phosphatidyl choline (PC) suggested that both the 1‐and 2‐forms of positional isomers were present. Hydrolysis of naturally occurring LPE and LPC with phospholipase C, and separation of the monoglycerides formed, confirmed the occurrence of two positional isomers of lysophosphatides.

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