Enzymatic RNA synthesis with deoxynucleoside 5'‐O‐(1‐thiotriphosphates)
- 2 January 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 315 (1) , 56-60
- https://doi.org/10.1016/0014-5793(93)81132-j
Abstract
We have investigated the incorporation of 2'‐deoxynucleoside‐5'‐O‐(1‐thiotriphosphates) into RNA transcripts using T7 RNA polymerase. With the exception of [α‐S]dGTP, we obtained full‐length transcripts of pre‐tRNAphe and pre‐tRNAtyr using an appropriate mixture of 2'‐deoxynucleoside 5'‐O‐(1‐thiotriphosphate) and the corresponding normal nucleoside triphosphate. The yields of the transcripts were comparable to those obtained with unmodified NTPs. Both substrates, [α‐S]dTTP and [α‐S]dATP, were inserted specifically. However, [α‐S]dCTP was excluded at specific sites. We could not obtain transcripts using the deoxyguanosine derivative.Keywords
This publication has 24 references indexed in Scilit:
- Important 2′-hydroxyl groups in model substrates for M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coliJournal of Molecular Biology, 1992
- Minimum ribonucleotide requirement for catalysis by the RNA hammerhead domainBiochemistry, 1992
- RNA tetraloops as minimalist substrates for aminoacylationBiochemistry, 1992
- Site-Specific Modification of Pre-mRNA: the 2′-Hydroxyl Groups at the Splice SitesScience, 1992
- Overlapping Nucleotide Determinants for Specific Aminoacylation of RNA MicrohelicesScience, 1992
- Differential effects of mutations in the protein and RNA moieties of RNase P on the efficiency of suppression by various tRNA suppressorsJournal of Molecular Biology, 1988
- Recent Excitement in Understanding Transfer RNA IdentityScience, 1988
- Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminatorsBiochemistry, 1985
- NUCLEOSIDE PHOSPHOROTHIOATESAnnual Review of Biochemistry, 1985
- Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reactionNature, 1983