Chromatin assembly on replicating DNAin vitro
Open Access
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (19) , 5767-5774
- https://doi.org/10.1093/nar/18.19.5767
Abstract
Replicating single-stranded DNA is preferentially assembled Into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histories H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract.This publication has 32 references indexed in Scilit:
- Negative supercoiling is not required for 5S RNA transcription in vitroCell, 1987
- H3.cntdot.H4 tetramer directs DNA and core histone octamer assembly in the nucleosome core particleBiochemistry, 1979
- A new procedure for purifying histone pairs H2A+H2B and H3+H4 from chromatin using hydroxylapatiteNucleic Acids Research, 1979
- A nuclear extract of xenopus laevis oocytes that accurately transcribes 5S RNA genesCell, 1978
- Assembly of newly replicated chromatinCell, 1978
- Preferential Association of Newly Synthesized H3 and H4 Histones with Newly Replicated DNA1The Journal of Biochemistry, 1978
- Specific folding and contraction of DNA by histones H3 and H4Cell, 1977
- The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneityNucleic Acids Research, 1977
- Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernelNucleic Acids Research, 1977
- Folding of the DNA double helix in chromatin-like structures from simian virus 40.Proceedings of the National Academy of Sciences, 1975