Measuring the in Vitro Susceptibility of Trichomonas vaginaIis to Mefronidazole

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Abstract
Strains of T. vaginalis were evaluated for in vitro responses to metronidazole. A serial 2-fold dilution method that measured minimal inhibitory concentrations (MIC) by cessation of trophozoite motility was compared with the disk broth method in which cessation of motility and inhibition of trophozoite proliferation were used for measurement of MIC. These methods gave comparable results in distinguishing a metronidazole-resistant and 2 metronidazole-susceptible strains of T. vaginalis under conditions of aerobic and anaerobic incubation. Anaerobic test conditions significantly lowered the MIC of metronidazole against a metronidazole-resistant strain. Clinical [human] isolates of T. vaginalis and strain ATCC 30001 from the American Type Culture Collection (Rockville, Maryland, USA) were tested. Two of 12 clinical isolates were resistant to metronidazole in vitro. The disk broth method is a simple and reliable screening method for detection of metronidazole-resistant strains of T. vaginalis.

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