A Variant of Tobacco Rattle Virus: Evidence for a Second Gene in RNA-2

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Abstract
The isolation of YS, a spontaneous variant of the CAM strain of tobacco rattle virus, is described. Unlike CAM, YS produced yellow symptoms in several species of host plant. In experiments with mixtures of virus particles from the 2 strains, this character was controlled by RNA-2. The coat proteins of YS and CAM were indistinguishable by serology, by electrophoresis in polyacrylamide gels or by tryptic peptide mapping. The mutation responsible for the yellow symptoms is not in the coat protein gene, but elsewhere in RNA-2.