Evaluation of magnetic alginate beads as a solid support for positive selection of CD34+ cells

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Abstract
Paramagnetic alginate beads with a 10–100 μm size range have been developed. These beads, when activated with chloroacetic anhydride and covalently coupled to avidin (30 μg/mg beads), were able to bind biotinylated goat anti-mouse (B-GAM) antibody (Ab). The beads with immobilized antibody were then used as a model system for the capture and non-enzymatic release of CD34+ (KG1a) cells. A maximum of 82% KG1a (average = 65 ± 16.1%) cell capture, and 57% (average = 51 ± 5.9%) cell release has been attained using this model system. Optimization of the system in terms of further bead size reduction, and in terms of developing a system to recover released cells with high purity, will make an excellent system for cell capture and nonenzymatic release. © 1994 John Wiley & Sons, Inc.