Can One Measure Rate Constants Using Chromatographic Methods?

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Abstract
It has recently been suggested that affinity chromotography can be used to measure rate constants from parameters determined from the elution curve. The analysis suggesting this possibility assumes that there is one type of reaction occurring and that steric factors can be ignored. In this paper a model is analyzed in which there can be a number of different binding sites. If one waits long enough, the resulting concentration profile tends toward a Gaussian form. This suggests that it would be experimentally very difficult to distinguish between single site and multiple site systems. Since steric factors undoubtedly operate, they would change the single site to the multiple site system. The implication of the analysis is that the proposed experiment does not necessarily measure the rate constants of interest.

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