Optimum Amino Acid Complement for Protein Synthesis by Rat Mammary Cells in Tissue Culture
Open Access
- 1 October 1976
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 59 (10) , 1758-1763
- https://doi.org/10.3168/jds.s0022-0302(76)84434-7
Abstract
A 3-dimensional central composite design utilizing 3 variables (X1 = lysine; X2 = methionine, valine and arginine; X3 = isoleucine, tryptophan, threonine, phenylalanine and histidine) at 5 concentrations each, was duplicated twice with mammary cells from lactating Sprague-Dawley rats. The optimum combination of amino acids for maximum milk protein synthesis for multiple regression models was X1 15.0-, X2 4.5- and X3 1.5-fold their quantities in Eagle''s minimal essential medium with leucine, tyrosine, cystine and glutamine at the base 1-fold in the medium.This publication has 11 references indexed in Scilit:
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