Transverse Gradient Electrophoresis: Protein Homogeneity Test and Subfractionation Technique
- 28 January 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 151 (3709) , 463-464
- https://doi.org/10.1126/science.151.3709.463
Abstract
Electrophoresis of protein (including enzyme) was conducted in a gel medium across which, transverse to the direction of protein migration, a continuous pH gradient extends. Any splitting of a resultant trace for the change of protein mobility with pH suggests both protein heterogeneity and the pH conditions under which further purification and subfractionation may best be pursued.This publication has 5 references indexed in Scilit:
- Subfractionation of Human Serum EnzymesScience, 1960
- An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individualsBiochemical Journal, 1959
- Multiple p H Levels in ChromatogramsScience, 1959
- Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adultsBiochemical Journal, 1955
- Separation and Concentration of Proteins in a pH Field Combined with an Electric FieldThe Journal of Chemical Physics, 1954