Carbachol‐induced reverse transformation of Chinese hamster ovary cells transfected with and expressing the m5 muscarinic acetylcholine receptor
- 13 March 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 245 (1-2) , 75-79
- https://doi.org/10.1016/0014-5793(89)80195-4
Abstract
Reverse transformation was induced in Chinese hamster ovary (CHO) cells transfected with and stably expressing the m5 subtype of the muscarinic acetylcholine receptor when stimulated with the muscarinic agonist, carbachol. Atropine, a muscarinic antagonist, blocked the carbachol-stimulated reverse transformation. CHO cells not transfected with the muscarinic receptor did not change with added carbachol. PMA induced reverse transformation without increasing cAMP accumulation in CHO cells. Carbachol, prostaglandin E2, and cholecystokinin increased cAMP accumulation but only carbachol caused reverse transformation. Carbachol-stimulated cAMP accumulation occurred at a higher concentration (EC50 10 μM) than did carbachol-stimulated reverse transformation (EC50 63 nM). Muscarinic m5 acetylcholine receptor transfected into CHO cells can induce reverse transformation which may be independent of cAMP.Keywords
This publication has 13 references indexed in Scilit:
- Stimulation of arachidonic acid release and inhibition of mitogenesis by cloned genes for muscarinic receptor subtypes stably expressed in A9 L cells.Proceedings of the National Academy of Sciences, 1988
- Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypesNature, 1988
- Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genesNeuron, 1988
- Specificity of the cAMP-induced gene exposure reaction in CHO cells.Proceedings of the National Academy of Sciences, 1988
- Phorbol esters and diacylglycerols amplify bradykinin-stimulated prostaglandin synthesis in Swiss 3T3 fibroblasts. Possible independence from protein kinase C.Journal of Biological Chemistry, 1988
- INOSITOL TRISPHOSPHATE AND DIACYLGLYCEROL: TWO INTERACTING SECOND MESSENGERSAnnual Review of Biochemistry, 1987
- The Role of Protein Kinase C in Transmembrane SignallingAnnual Review of Cell Biology, 1986
- CHANGES IN SURFACE MORPHOLOGY OF CHINESE HAMSTER OVARY CELLS DURING THE CELL CYCLEThe Journal of cell biology, 1973
- Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and TestosteroneProceedings of the National Academy of Sciences, 1971