The α-Mannosidase fromCanavalia ensiformisSeeds: Chemical and Kinetic Properties and Action on Animal Lymphocytes
- 1 January 1988
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 369 (1) , 165-170
- https://doi.org/10.1515/bchm3.1988.369.1.165
Abstract
The .alpha.-mannosidase from Canavalia ensiformis was characterized with repect to molecular mass, glycoprotein nature, amino-acid composition, enzymic properties and action on animal cells. The enzyme is composed of two pairs of subunits (molecular mass 44 and 66 kDa) which form a tetramer (220 kDa). The larger subunit is glycosylated, the smaller one is not. Both subunits have similar amino-acid compositions. The larger subunit contains a surplus of alanine, aspartic acid/asparagine, histidine, phenylalanine and tryrosine, the smaller one a surplus of glutamic acid/glutamine, serine and threonine. The enzyme is subject to product inhibition by mannose. It stimulates the proliferation of B-lymphocytes from nude mice.This publication has 26 references indexed in Scilit:
- Mercaptoethanol Assists in Cleaving a Cysteine/Cystine-Free Protein into its SubunitsBiological Chemistry Hoppe-Seyler, 1986
- Interaction of the α-Mannosidase fromCanavalia ensiformiswith the Lectin from the Same Plant, Concanavalin ABiological Chemistry Hoppe-Seyler, 1986
- Isolation of theCanavalia ensiformisSeed α-Mannosidase by Chromatography on Concanavalin A, the Lectin from the Same Plant, without Involving its Sugar Binding SiteBiological Chemistry Hoppe-Seyler, 1986
- Interactions between Lectins and other Components of Leguminous Protein BodiesBiological Chemistry Hoppe-Seyler, 1985
- A new Monooxygenase Product from 7-Ethoxycoumarin and its Relation to the O-Dealkylation ReactionBiological Chemistry Hoppe-Seyler, 1985
- Assessment of protein sequence identity from amino acid composition dataJournal of Theoretical Biology, 1977
- Induction of Lymphocyte Transformation by Sequential Treatment with Neuraminidase and Galactose OxidaseProceedings of the National Academy of Sciences, 1973
- Isolation and Characterization of α-D-Mannosidase from Soy BeanThe Journal of Biochemistry, 1971
- Mannosidase, glucosidase, and galactosidase in sweet almond emulsinArchives of Biochemistry and Biophysics, 1970
- Studies on the Glycosidases in Jack Bean MealPublished by Elsevier ,1967