Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA

Publisher Website
Google Scholar
Abstract
A thermo-acidophilic Gram-positive bacterium, strain CsHg2T, which grows aerobically at 35–65 °C (optimum 55 °C) and at pH 2·0–6·0 (optimum 4·0), was isolated from a geothermal pool located in Coso Hot Springs in the Mojave Desert, California, USA. Phylogenetic analysis of 16S rRNA gene sequences showed that this bacterium was most closely related to the type strains ofAlicyclobacillus acidocaldarius(97·8 % identity) andAlicyclobacillus sendaiensis(96·9 %), three Japanese strains denoted as UZ-1, KHA-31 and MIH 332 (96·1–96·5 %) andAlicyclobacillusgenomic species FR-6 (96·3 %). Phenotypic characteristics including temperature and pH optima, G+C composition, acid production from a variety of carbon sources and sensitivity to different metal salts distinguished CsHg2TfromA. acidocaldarius,A. sendaiensisand FR-6. The cell lipid membrane was composed mainly ofω-cyclohexyl fatty acid, consistent with membranes from otherAlicyclobacillusspecies. Very low DNA–DNA hybridization values between CsHg2Tand the type strains ofAlicyclobacillusindicate that CsHg2Trepresents a distinct species. On the basis of these results, the nameAlicyclobacillus vulcanalissp. nov. is proposed for this organism. The type strain is CsHg2T(ATCC BAA-915T=DSM 16176T).

This publication has 24 references indexed in Scilit: