Studies on benzo[a]pyrene mono-oxygenase in the shore crabCarcinus maenas

Abstract
A cytochrome P-450-dependent benzo[a]pyrene mono-oxygenase enzyme system (BPM) has been identified and partially characterized in males of the shore crabCarcinus maenas(L.). Apparent Kmvalues obtained at 30 °C using microsomal preparations from the antennary glands of animals collected during summer were in the range 1.61–2.11µM. The cytochrome P-450 content was 0·022 nmol/mg microsomal protein when BPM activity in the same preparation was 0·085 nmol/mg protein/min.

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