Calcium-Controlled Secretion Is Effected through a Guanine Nucleotide Regulatory Protein in Parathyroid Cells
- 1 December 1986
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 119 (6) , 2700-2703
- https://doi.org/10.1210/endo-119-6-2700
Abstract
Calcium regulates parathyroid secretion through mechanisms yet to be elucidated. We have investigated this phenomenon through use of pertussis toxin which catalyzes ADP-ribosylation and inactivation of a guanine nucleotide regulatory protein, possibly Ni or No. Calcium inhibition of PTH release is blocked in cells treated with pertussis toxin, and there is concomitant ADP-ribosylation of a 40-kilodalton protein. The ionophore A23187 inhibits secretion in toxin-treated as well as in control cells. We conclude that a guanine nucleotide regulatory protein is involved in calcium regulation of PTH secretion at a locus proximal to the intracellular site effecting inhibition of secretion.This publication has 9 references indexed in Scilit:
- Antibodies against the carboxyl-terminal 5-kDa peptide of the alpha subunit of transducin crossreact with the 40-kDa but not the 39-kDa guanine nucleotide binding protein from brain.Proceedings of the National Academy of Sciences, 1985
- Radioimmunoassay for the middle region of human parathyroid hormone: comparison of two radioiodinated synthetic peptidesClinica Chimica Acta; International Journal of Clinical Chemistry, 1985
- Pertussis toxin inhibits the rise in the intracellular concentration of free calcium that is induced by chemotactic factors in rabbit neutrophils: Possible role of the “G proteins” in calcium mobilizationBiochemical and Biophysical Research Communications, 1984
- Dual mechanisms involved in development of diverse biological activities of islet-activating protein, pertussis toxin, as revealed by chemical modification of lysine residues in the toxin moleculeBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Relationship between parathyroid hormone secretion and cytosolic calcium concentration in dispersed bovine parathyroid cells.Proceedings of the National Academy of Sciences, 1984
- Characterization of the Dopamine-Responsive Adenylate Cyclase of Bovine Parathyroid Cells and Its Relationship to Parathyroid Hormone SecretionEndocrinology, 1980
- Effects of the Calcium Ionophore A23187 on Dispersed Bovine Parathyroid CellsEndocrinology, 1980
- Preparation of Viable Isolated Bovine Parathyroid CellsEndocrinology, 1976
- RELATIONSHIP OF A LOW BLOOD CALCIUM TO PARATHYROID SECRETIONEndocrinology, 1942