Solubilized candida cell Wall β‐glucan, CSBG, is an epitope of natural human antibody
- 1 February 2003
- journal article
- research article
- Published by Wiley in Drug Development Research
- Vol. 58 (2) , 179-189
- https://doi.org/10.1002/ddr.10158
Abstract
We have recently developed a protocol to obtain a soluble Candida spp. β1, 3‐glucan (CSBG) by sodium hypochlorite (NaClO) oxidation and subsequent dimethyl sulfoxide (DMSO) extraction. CSBG is composed mainly of β‐1,3 and β‐1,6‐glucosidic linkages with a small amount of branching. Antibody to β‐glucan is generally difficult to produce. In the present study, the specificity of the human sera to CSBG was examined by ELISA. Using CSBG‐coated plates, sera from normal human volunteers showed high reactivity that was neutralized by adding soluble CSBG as a competitor. However, the reactivity could not be neutralized by a β‐1,6 branched β‐1,3‐glucan, grifolan. Similar specificity of the immunoglobulin (Ig) was detected in commercially available γ‐globulin prepared from pooled human sera. Comparing various standard glucans, a major epitope was present on β‐1,6 glucan segment. Drug Dev. Res. 58:179–189, 2003.Keywords
This publication has 33 references indexed in Scilit:
- Toll‐like receptors: molecular mechanisms of the mammalian immune responseImmunology, 2000
- Toll-like receptors in the induction of the innate immune responseNature, 2000
- CD14, new aspects of ligand and signal diversityMicrobes and Infection, 2000
- The Wall Structure of Schizosaccharomyces pombeMicrobiology, 2000
- Host resistance to infection: genetic control of lipopolysaccharide responsiveness by Toll-like receptor genesTrends in Genetics, 1999
- Solubilization of yeast cell-wall β-(1→3)-d-glucan by sodium hypochlorite oxidation and dimethyl sulfoxide extractionCarbohydrate Research, 1999
- Architecture of the Yeast Cell WallJournal of Biological Chemistry, 1997
- A (1→3,1→4)‐β‐glucan‐specific monoclonal antibody and its use in the quantitation and immunocytochemical location of (1→3,1→4)‐β‐glucansThe Plant Journal, 1994
- The molecular structures of some glucans from the cell walls of Schizosaccharomyces pombeCarbohydrate Research, 1977
- The structure of a β-(1→6)-d-glucan from yeast cell wallsBiochemical Journal, 1973