EFFECTS OF HEPARIN ON ALIMENTARY HYPERLIPEMIA. AN ELECTROPHORETIC STUDY 1

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Abstract
The effects of intraven. admd. heparin on plasma proteins and lipids in alimentary hyperlipemia were studied in 8 normals by paper electrophoresis and moving boundary electrophoresis. After injn. of heparin analysis of the serum by paper electrophoresis with subsequent staining for proteins and lipids revealed that the lipid band, normally present in the beta globulin area, was then in the area of alpha-2 or alpha-1 globulin. Instead of the lipid band, normally present in the alpha-1 globulin area, lipid material migrated ahead of the albumin. The lipid band, on the lipid stained paper strip, which appeared near the starting line in the alimentary hyperlipemic state due to the chylomicra, disappeared. Moving boundary electrophoresis showed in the alimentary hyperlipemic state that the chylomicra moved with mobility of alpha-2 globulins. After injn. of heparin there was a decrease in beta-1 globulin peak and an increase in alpha-2 or alpha-1 globulin peak or both. A pre-albumin component was also observed. This pre-albumin component appeared after 10 minutes of an electrophoretic expt. and after 40 minutes had moved beyond the visual field. Storage of fasting and hyperlipemic serum for 3 - 4 weeks in a refrigerator gave paper electrophoretic patterns similar to those after an injection of heparin. On the lipid stained paper strip a pre-albumin component was seen, and the beta globulin lipid material migrated with the speed of the alpha globulins. Injn. of heparin during a fasting state showed no changes in the electrophoretic patterns.

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