Proliferation of Mammalian Cells in Ion-Controlled Environments2

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Abstract
Tissue-culture and nuclei-enumeration procedures were used to study the effects of the aerial net space charge, ion concentration and polarity, on the proliferation rates of mammalian cells in vitro. Cells were grown in T-60 flasks containing 15 ml of culture medium, gassed with CO2 in air, and incubated at 37.5° C. The aerial environment within the culture flasks was altered by a tritium ion generator within the flask. Cultures grown in an atmosphere in which negative ions predominated showed a significantly greater proliferation rate than their accompanying controls, whereas those grown in a positively ionized atmosphere were retarded in proliferation rate to an even more significant degree. A second-transplant generation, though grown in a nonionized atmosphere, tended to retain these altered rates of proliferation originally induced by air ionization. A relationship to similar contemporary physiological experiments is indicated and a possible mechanism for the characteristic biological effects of air ions is cited.

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