A Theory of Stepwise Elution Chromatography with Small Sample Loads: Effect of the Top of the Column
- 1 April 1982
- journal article
- Published by Taylor & Francis in Separation Science and Technology
- Vol. 17 (5) , 695-716
- https://doi.org/10.1080/01496398208068561
Abstract
A theory of stepwise elution chromatography was developed on the basis of a continuity equation for a simplest case of linear chromatography with small sample loads. Longitudinal diffusions taken into consideration are limited to both thermal Brownian diffusion occurring in the mobile phase and diffusion caused by the flow heterogeneity. However, account is taken of the existence of the column top, where longitudinal Brownian diffusion can be assumed to occur associated with a diffusion that is provoked by a type of flow heterogeneity. This paper provides a fundamental theory that was necessary for the earlier development of the theory of gradient hydroxyapatite chromatography.Keywords
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