Two-Step Red-Mediated Recombination for versatile High-Efficiency Markerless DNA Manipulation in Escherichia Coli
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Open Access
- 1 February 2006
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 40 (2) , 191-197
- https://doi.org/10.2144/000112096
Abstract
Red recombination using PCR-amplified selectable markers is a well-established technique for mutagenesis of large DNA molecules in Escherichia coli. The system has limited efficacy and versatility,... BAC Recombineering Straightforward modification of BAC-cloned sequences is critical for generating transgenic animals and for functional studies of DNA viruses. Although a number of methods for mar...Keywords
Funding Information
- National Institutes of Health (AI060843-02)
- United States Department of Agriculture NRI program (CSREES 2003-02234)
- Morris Animal Foundation (D03EQ-74)
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