IR−MALDI−LDI Combined with Ion Mobility Orthogonal Time-of-Flight Mass Spectrometry

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Abstract
Most MALDI instrumentation uses UV lasers. We have designed a MALDI−IM−oTOF−MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, λ = 2.8−3.2 μm at 20 Hz) to perform IR−LDI or IR−MALDI and a Nd:YLF laser (Crystalaser, λ = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined “trend lines”. Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR−LDI produced similar results as IR−MALDI for the direct tissue analysis of phospholipids from rat brain. Keywords: ion-mobility MALDI • UV lasers • IR lasers • adducts reduction

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