Gene mapping and linkage analysis in Chinese hamster: Assignment of the genes forAPRT, LDHA, IDH2, andGAA to chromosome 3
- 1 July 1983
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 9 (4) , 477-487
- https://doi.org/10.1007/bf01543048
Abstract
Interspecific somatic cell hybrids were generated by fusion of Chinese hamster spleen cells or primary fibroblasts with cells from an adenine phosphoribosyltransferase (APRT)-deficient mouse subline derived from LMTK− Cl.1D. Subclones which had been selected for either retention or loss ofAPRT were subjected to combined isozyme and chromosome segregation analysis. Concordant expression or segregation of Chinese hamster APRT, lactate dehydrogenase A (LDHA), isocitrate dehydrogenase 2 (IDH2), and α-glucosidase (GAA) with Chinese hamster chromosome 3 allowed provisional assignment of all four loci to that chromosome. Exceptional subclones, in which coordinate segregation of these syntenic markers was disrupted by chromosome breakage or deletions, allowed further localization of these genes to specific regions of the 3 chromosome.This publication has 32 references indexed in Scilit:
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