Detection of Anti-HeLa Antibodies in Rabbit Antiserum by Indirect Hemagglutination

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Abstract
This report describes methodology of an indirect hemagglutination test used for detection of anti-HeLa antibodies in serum of immunized rabbits, and describes preparation of a sensitizing antigen from HeLa cells. Material obtained from sonically disrupted HeLa cells by extraction of trichloracetic-acid precipitate with buffered saline was used to sensitize tanned sheep erythrocytes. Sensitized erythrocytes were agglutinated to high titer by anti-HeLa rabbit serum, but minimally by normal rabbit serum. Reactivity of the sensitizing extract was considerably reduced by treatment of HeLa cells with tannic acid prior to disruption and extraction.