Electrowetting-Based Microfluidics for Analysis of Peptides and Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
- 3 July 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (16) , 4833-4838
- https://doi.org/10.1021/ac0498112
Abstract
A new technique for preparing samples for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is reported. The technique relies on electrowetting-on-dielectric (EWOD) to move droplets containing proteins or peptides and matrix to specific locations on an array of electrodes for analysis. Standard MALDI-MS reagents, analytes, concentrations, and recipes are demonstrated to be compatible with the technique. Mass spectra are comparable to those collected by conventional methods. Nonspecific adsorption of analytes to device surfaces is demonstrated to be negligible. The results suggest that EWOD may be a useful tool for automating sample preparation for high-throughput proteomics and other applications of MALDI-MS.Keywords
This publication has 21 references indexed in Scilit:
- Improved detection of higher molecular weight proteins by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry on polytetrafluoroethylene surfacesRapid Communications in Mass Spectrometry, 2003
- Low voltage electrowetting-on-dielectricJournal of Applied Physics, 2002
- Chemical reactions in microdroplets by electrostatic manipulation of droplets in liquid mediaLab on a Chip, 2002
- Ready‐made matrix‐assisted laser desorption/ionization target plates coated with thin matrix layer for automated sample deposition in high‐density array formatRapid Communications in Mass Spectrometry, 2001
- Amorphous fluoropolymers as insulators for reversible low-voltage electrowettingJournal of Applied Physics, 2001
- The Sequence of the Human GenomeScience, 2001
- Signal Amplification Using “Spot-on-a-Chip” Technology for the Identification of Proteins via MALDI-TOF MSAnalytical Chemistry, 2000
- Electrowetting-based actuation of liquid droplets for microfluidic applicationsApplied Physics Letters, 2000
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988
- Protein and polymer analyses up to m/z 100 000 by laser ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1988