A System for the Simultaneous Chemical Synthesis of Different DNA Fragments on Solid Support
- 1 October 1986
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 5 (5) , 413-419
- https://doi.org/10.1089/dna.1986.5.413
Abstract
A mechanical apparatus for the simultaneous synthesis of different DNA fragments on solid support is described. This new system allows the use of different kinds of supports and is amenable to automation. Utilizing this apparatus the synthesis of DNA fragments can be speeded up considerably. The effectiveness of the system is demonstrated by the simultaneous synthesis of DNA fragments up to 36 nucleotides long using phosphoamidites as building blocks and controlled pore glass as solid support.This publication has 16 references indexed in Scilit:
- A simple and rapid method for sequencing DNAFEBS Letters, 1985
- Filter disc supported oligonucleotide synthesis by the phosphite methodNucleic Acids Research, 1984
- Improved synthesis of oligodeoxynucleotides on controlled pore glass using phosphotriesther chemistry and a flow systemTetrahedron Letters, 1983
- Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mersJournal of the American Chemical Society, 1983
- An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotidesTetrahedron Letters, 1983
- Transient protection: efficient one-flask syntheses of protected deoxynucleosidesJournal of the American Chemical Society, 1982
- New effective method for the synthesis of oligonucleotides via phosphotriester intermediatesNucleic Acids Research, 1982
- Synthesis of oligodeoxyribonucleotides on silica gel supportNucleic Acids Research, 1981
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Studies on Polynucleotides. XXIV.1 The Stepwise Synthesis of Specific Deoxyribopolynucleotides (4).2 Protected Derivatives of Deoxyribonucleosides and New Syntheses of Deoxyribonucleoside-3″ Phosphates3Journal of the American Chemical Society, 1963