METHOTREXATE-LEUCOVORIN SYNCHRONIZATION OF HUMAN-BONE MARROW CULTURES - INDUCTION OF HIGH-RESOLUTION R-BANDING AND G-BANDING

  • 1 January 1985
    • journal article
    • research article
    • Vol. 5  (2) , 171-172
Abstract
Simple R-banding and G-banding techniques based on a methotrexate S-phase block, released by exposure to leucovorin, were used for preparation of human bone marrow samples. R-bands were induced by adding 5-bromodeoxyuridine and Hoechst 33258 to the cultures, followed by a modified FPG-staining. G-bands were induced by adding ethidium bromide to the cultures, followed by a modified trypsin treatment of the slides. Due to Hoechst 33258 or ethidium bromide exposure prior to harvest, chromosome contraction was partly inhibited and well-defined R-banding or G-banding was observed after appropriate staining in elongated prometaphase chromosomes.