Removal of repeated sequences from hybridisation probes
Open Access
- 25 March 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (6) , 1905-1922
- https://doi.org/10.1093/nar/13.6.1905
Abstract
Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.Keywords
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