Localization of DNA sequences promoting RNA polymerase I activity in Drosophila.
- 1 June 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (11) , 3265-3268
- https://doi.org/10.1073/pnas.80.11.3265
Abstract
BAL-31 nuclease was used to delete sequences that surround the transcription initiation site of Drosophila ribosomal DNA. A series of deletions was used as templates for in vitro transcription in a Drosophila cell-free system to identify sequences that influence the activity of RNA polymerase I. Sequences that lie upstream of the site of transcription initiation (nucleotide +1) affect rRNA synthesis. The major promoter of polymerase I involves the sequence -43 to -27 and the region between nucleotides -18 and +20 contains sequences capable of sustaining a low level of accurate transcription.Keywords
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