Effect of wastewater from olive oil mills on nitrogenase activity and growth of Azotobacter chroococcum

Publisher Website
Google Scholar
Abstract
The effect of 1, 5, 10, 15, and 20% (v/v) of wastewater from olive oil mills (“alpechin”) was investigated in cells of Azotobacter chroococcum grown in chemically defined media (N‐free or with NH+4) and dialyzed‐soil media. Wastewater from olive oil mills at concentrations of 1 to 20% (v/v) significantly reduced dinitrogen fixation and growth of Azotobacter chroococcum in chemically defined N‐free medium, whereas the presence of 1 to 15% had a stimulatory effect on dinitrogen fixation and growth of Azotobacter in dialyzed‐soil medium. Azotobacter chroococcum grew on NH+4 media (without glucose) amended with alpechin, suggesting that these wastes were utilized by Azotobacter as a carbon source.