USE OF 5-BRDURD/313 NM LIGHT TECHNIQUE FOR IDENTIFICATION OF CELLS IN INITIAL S-PHASE WITHIN GM-CFCC COMPARTMENT

  • 1 January 1983
    • journal article
    • research article
    • Vol. 11  (5) , 402-409
Abstract
Long-term, low-level, BrdUrd [5-bromodeoxyoxidine] infusion identifies 2 subpopulations of GM-CFCc [granulocyte-macrophage progenitor cells] with quite dissimilar sensitivities to 313 nm light. The responses of these 2 GM-CFCc subpopulations to hydroxyurea indicate that both are rapidly proliferating at the time of the assay. The absolute UV-light sensitivity of the S-phase components and the effects of increasing BrdUrd concentration indicate that the 2 GM-CFCc subpopulations passed through the previous cell cycle at widely disparate rates. Further, those GM-CFCc originating from a parental cell with a slow turnover are associated with a lower buoyant density than those GM-CFCc that were in rapid cycle for at least 2 generations. The resistance to 313 nm-light irradiation, shown by S-phase cells in the 1st cell cycle of the BrdUrd labeling, may provide evidence of the proliferative history of the cell being assayed.