Expression of Water Channel mRNA Following Cerebral Ischemia
- 1 January 2000
- book chapter
- Published by Springer Nature
- Vol. 76, 239-241
- https://doi.org/10.1007/978-3-7091-6346-7_48
Abstract
Water channel is a protein which regulates transcellular water permeability. Among mRNA expression of six principal mammalian water channels, AQ4 mRNA expression was highest in the brain. Water channels are supposed to regulate cerebral edema but the detailed physiological and pathological function is unknown. Brain edema has been analyzed as an aspect of ion channel injury or membrane injury. However the transportation of water molecule itself following cerebral ischemia is unknown. As water channels transport only water molecules, the functional changes of water channels following cerebral ischemia are of great interest. To evaluate the role of water channels in cerebral edema following cerebral ischemia, the changes of water channel mRNA expression were evaluated. Cerebral edema was induced by suture method. The extraction of water channel mRNA was performed according to Chomczynsli and Sacchi. RT-PCR was applied to extracted mRNA. Water channel mRNA electrophoresis was performed. For semiquantified evaluation of water channel, mRNA intensities of the infarct hemisphere and normal hemisphere were compared. The expression of water channel mRNA was decreased following cerebral ischemia. This damage leads to loose physiological control of water permeability of the cell membrane in the neuron, glia and endothelial cells which leads to brain edema.Keywords
This publication has 17 references indexed in Scilit:
- Apoptosis in the striatum of rats following intraperitoneal injection of 3-nitropropionic acidBrain Research, 1997
- Pathophysiology of the Aquaporin Water ChannelsAnnual Review of Physiology, 1996
- Quantitative Analysis of Aquaporin mRNA Expression in Rat Tissues by RNase Protection AssayDNA and Cell Biology, 1996
- The CHIP28 water channel visualized in ice by electron crystallographyNature Structural & Molecular Biology, 1995
- Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance.Proceedings of the National Academy of Sciences, 1994
- Projection Structure of the CHIP28 Water Channel in Lipid Bilayer Membranes at 12-.ANG. ResolutionBiochemistry, 1994
- Concurrent expression of erythroid and renal aquaporin CHIP and appearance of water channel activity in perinatal rats.Journal of Clinical Investigation, 1993
- Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.Proceedings of the National Academy of Sciences, 1991
- Brain injury, edema, and vascular permeability changes induced by oxygen‐derived free radicalsNeurology, 1984
- Experimental cerebral ischemia in Mongolian gerbilsActa Neuropathologica, 1976