Conditional Gene Knockout Using Cre Recombinase
- 1 January 2001
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 17 (3) , 269-276
- https://doi.org/10.1385/mb:17:3:269
Abstract
Cre recombinase has become an important instrument for achieving precise genetic manipulation in mice. Many of these desired genetic manipulations rely on Cre’s ability to direct spatially and temporally specified excision of a predesignated DNA sequence that has been flanked by directly repeated copies of the loxP recombination site. Success in achieving such conditional mutagenesis in mice depends both on the careful design of conditional alleles and on reliable detection of cre gene expression. These procedures include PCR, immunohistochemistry and the use of a recombination-proficient GFP-tagged Cre protein.Keywords
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