Uniquely Regulated Proteinase Inhibitor I Gene in a Wild Tomato Species
Open Access
- 1 October 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 97 (2) , 496-501
- https://doi.org/10.1104/pp.97.2.496
Abstract
A uniquely regulated proteinase inhibitor I gene was isolated from the wild tomato species Lycopersicon peruvianum (L.) Mill. (LA 107) and characterized. The inhibitor gene is wound-inducible in leaves and is expressed in unripe fruit during development. The gene (λ clone 1) is present on a 15.5 kilobase pair Sal 1-SalI genomic DNA fragment. Southern blot analysis of L. peruvianum genomic DNA shows only one strongly hybridizing DNA fragment to probes derived from λ clone 1. S1 nuclease protection experiments and Northern analyses confirm that this gene is both wound-inducible in leaves and developmentally regulated in young unripe fruit. These observations are supported by comparisons of the 5′-flanking DNA sequences of the L. peruvianum inhibitor I gene with known elicitor responsive cis-acting sequences. The transcriptional regulation of the λ clone 1 inhibitor I gene in leaves of wounded plants and in developing unripe fruit indicates that the gene contains unique complex regulating elements. These elements respond to both environmental and developmental tissue-specific signals that can regulate proteinase inhibitor synthesis to protect the tissues of this wild species of tomato against predators and pathogens.Keywords
This publication has 11 references indexed in Scilit:
- A novel fruit-expressed trypsin inhibitor I gene from a wild species of tomato.Journal of Biological Chemistry, 1991
- Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae.Proceedings of the National Academy of Sciences, 1989
- ISOLATION AND CHARACTERIZATION OF A NOVEL, DEVELOPMENTALLY REGULATED PROTEINASE INHIBITOR-I PROTEIN AND CDNA FROM THE FRUIT OF A WILD-SPECIES OF TOMATO1989
- Proteinase inhibitor gene families: Strategies for transformation to improve plant defenses against herbivoresBioEssays, 1989
- Ethylene-regulated expression of a tomato fruit ripening gene encoding a proteinase inhibitor I with a glutamic residue at the reactive site.Proceedings of the National Academy of Sciences, 1988
- Wound-inducible expression of a potato inhibitor II-chloramphenicol acetyltransferase gene fusion in transgenic tobacco plantsProceedings of the National Academy of Sciences, 1987
- Molecular characterization and phylogenetic studies of a wound-inducible proteinase inhibitor I gene in Lycopersicon species.Proceedings of the National Academy of Sciences, 1986
- WOUND-INDUCED PROTEINASE-INHIBITORS FROM TOMATO LEAVES .1. THE CDNA-DEDUCED PRIMARY STRUCTURE OF PRE-INHIBITOR-I AND ITS POST-TRANSLATIONAL PROCESSING1985
- In vitro synthesis of pre-proteins of vacuolar compartmented proteinase inhibitors that accumulate in leaves of wounded tomato plantsProceedings of the National Academy of Sciences, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977