Skin Storage in Liquid Nitrogen

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Abstract
The problem of cell survival as well as the biochemical and histological effects on human skin of low temperature storage in liquid N has received much attention. However, little is known of the submicroscopic alterations induced by such treatment. Rapid or slow freezing, direct or mediate contact with the liquid N, pretreatment before freezing, type and concentration of cryoprotectants all have various effects on the fine morphology of epidermal cells. Glycerol has better cryoprotective activity than DMSO [dimethyl sulfoxide] at concentrations of 15% or less. All available reports on the problem of cryopreservation and the use of cryoprotectants were examined.