CYTOGENETIC AND MORPHOGENETIC CHANGES IN OOGENESIS AND EMBRYOGENESIS INDUCED BY PYRIMETHAMINE AND 2,4,5-TRICHLOROPHENOXYACETIC ACID IN MEIOTIC OOCYTES OF ALBINO-RATS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 16  (7) , 1220-1227
Abstract
The effect of pyrimethanime (chloridin) and 2,4,5-T on embryo survival, cleavage rate and frequency of chromosome aberrations when injected into female rats just before ovulation was studied. Pyrimethamine induced 70% of embryonic death at a dose of 5 mg/kg and 27% at a dose of 2 mg/kg. A dose of 200 mg/kg of 2,4,5-T had no embryotoxic effect, but 400 mg/kg increased the embryo loss up to 48%. Pyrimethamine (5 mg/kg) and 2,4,5-T (400 mg/kg), injected intragastrically just before ovulation, significantly affected the cleavage rate in the 4 day rat embryos. The frequency of chromosome aberrations in ovulated oocytes at metaphase, II subjected to pyrimethamine and 2,4,5-T, reached 6-8% and exceeded the control value in 5-10 times.