Hydroxyurea: Effect On Growth, Structure, and [3H]Thymidine Uptake of Trypanosoma Brucei Procyclic Culture Forms
- 1 February 1980
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 27 (1) , 122-128
- https://doi.org/10.1111/j.1550-7408.1980.tb04241.x
Abstract
SYNOPSIS Hydroxyurea (HU) concentrations above 50 μg/ml reversibly inhibited cell division of Trypanosoma brucei brucei stock STIB 366 procyclic culture forms, but not growth of individual cells. the volume of the nucleus and of the cytoplasm increased in the presence of the drug as did the percentage of cells with 2 kinetoplasts. Electron microscopy revelaed that the nuclear membrane was extended forming protrusions which surrounded areas of the cytoplasm. Replication of the kinetoplast DNA did not seem to be affected by HU. the uptake of [3H]thymidine increased in the presence of 25 μ/ml HU 3‐fold compared to control cells. the nuclear volume and the dry weight of the culture forms increased proportionally to the amount of label incorporated.Keywords
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