A comparison of two phage coat protein-RNA interactions
Open Access
- 1 January 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (11) , 5055-5066
- https://doi.org/10.1093/nar/16.11.5055
Abstract
The interaction between the coat protein of the group I bacteriophage fr with its translational operator site is compared with the previously studied R17 interaction. The sequence of the two RNA binding sites differ by 2 of 20 nucleotides and two coat proteins by 17 of 129 amino acids. An analysis of the binding of fr coat protein to 24 operator variants revealed that the two proteins recognize operator sequences in virtually the same way. However, fr coat protein binds to nearly every RNA 6 to 14-fold tighter than R17 coat protein. Since the fr operator is a weaker binding variant and the fr coat protein shows a different temperature dependence of binding, it is unlikely that the two systems have different Kas in vivo. RNA fragments containing the operator sequences can initiate the capsid assembly with both fr and R17 coat protein. Surprisingly, the two coat proteins can form a mixed capsid in vitro.This publication has 11 references indexed in Scilit:
- Role of a bulged A residue in a specific RNA-protein interactionBiochemistry, 1987
- RNA binding site of R17 coat proteinBiochemistry, 1987
- An RNA mutation that increases the affinity of an RNA–protein interactionNucleic Acids Research, 1987
- Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templatesNucleic Acids Research, 1987
- Interaction of R17 coat protein with synthetic variants of its ribonucleic acid binding siteBiochemistry, 1983
- Kinetic and thermodynamic characterization of the R17 coat protein-ribonucleic acid interactionBiochemistry, 1983
- Enzymic synthesis of a 21-nucleotide coat protein binding fragment of R17 ribonucleic acidBiochemistry, 1982
- [Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments].1982
- The regulatory region of phage fr replicase cistron. III. Initiation activity of specific fr RNA fragmentsNucleic Acids Research, 1982
- Pentalysine-deoxyribonucleic acid interactions: a model for the general effects of ion concentrations on the interactions of proteins with nucleic acidsBiochemistry, 1980