DIPETALONEMA-VITEAE (FILARIOIDEA) - DEVELOPMENT OF THE INFECTIVE LARVAE INVITRO
- 1 January 1981
- journal article
- research article
- Vol. 38 (3) , 241-249
Abstract
The development of D. viteae 3rd stage larvae was attempted in vitro. A monophasic culture system consisting of BHK-21 [baby hamster kidney] medium supplemented with 10% tryptose-phosphate broth and 15% fresh jird serum allowed the growth of previously in vivo triggered larvae, but not of those isolated from ticks. The larvae could complete the 3rd moult and grew on as 4th stage larvae up to 5 mm. Development was comparable to that observed in vivo. The presence of an irradiated hamster kidney cell feeder layer could replace an in vivo trigger. The development of the infective larvae took place up to the 3rd moult. Some larvae completed the moult to the 4th stage and reached a maximum length of 3 mm after 14 days. These culture systems offer the possibility to study in vitro the complete development of the infective larvae to 4th stage larvae.This publication has 8 references indexed in Scilit:
- Thein vitrocultivation of the infective larvae and the early mammalian stages of the filarial worm,Brugia pahangiPathogens and Global Health, 1979
- The isolation and partial characterization of a protective antigen from developing larvae of Ascaris suumInternational Journal for Parasitology, 1979
- STUDIES ON DIPETALONEMA-VITEAE (FILARIOIDEA) .3. ANTIBODY-DEPENDENT CELL-MEDIATED DESTRUCTION OF MICROFILARIAE INVIVO1979
- Development ofDipetalonema viteae third-stage larvae (Nematoda: Filarioidea) in micropore chambers implanted into jirds, hamsters, normal and immunized miceZeitschrift Fur Parasitenkunde-Parasitology Research, 1979
- Ascaris suum: Immunization with soluble antigens in the guinea pigInternational Journal for Parasitology, 1977
- Development of larvae of Ascaris suum from the third to the fourth stage in a chemically defined mediumInternational Journal for Parasitology, 1977
- Studies on filariasis. III. Partial growth of the mammalian stages of Dirofilaria immitis in vitroExperimental Parasitology, 1964
- Dipetalonema witei, Filarial Parasite of the Jird, Meriones libycus. I. Maintenance in the LaboratoryJournal of Parasitology, 1961