Immunochemically-active cross-linked polystyrene preparations

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Abstract
Highly swollen linear chains of polystyrene have been cross-linked by means of antibody protein, by using diazo coupling and coupling via the sulfhydryl group of the protein by means of a mercury atom. Preparations containing 15% to over 60% of protein, by weight, are described. Such products are able selectively to adsorb their appropriate antigen from solution. With ovalbumin and anthrax protective antigens the action is markedly specific, only small quantities of heterologous protein being adsorbed. With the Pasteurella pestis toxin, non-specific adsorption is substantial. With the anthrax preparation, the antigen, when adsorbed on the polymer, retains its biological competence as judged by its ability to stimulate antibody production and to produce immunity to challenge in animals after injection.